After Karen's newest wish took effect, all the girls in the cheerleading squad (that were practicing that day) became guys, but they still acted like girls.
After practice was over, Jon walked over to who once was Sarah, but who now called himself Seth. They both talked to each other about girlish things, giggling like girls, then walked back to the locker room.
But just as Jon passed over the threshold of the entrance to the girl's locker room (which is accessible from the outside of the school), he stopped acting like a girl and took notice of the cheerleader outfit he had on. Apparently, when he's at school, he's his normal self. But when he's OUTSIDE of the school, he's the way Karen wished him to be that morning.
He turned around to go back outside to find Karen, when he saw the rest of the changed cheerleaders entering the room. They all ceased acting like girls and were immensely shocked to find that they were wearing girl's clothing.
"What the hell am I wearing?!" yelled Seth.
It seemed that whatever happened to Jon, happened to the rest of them as well.