Jon no longer had the desire to wear the wishing panties, since that only applies to the person wearing them--and he wasn't wearing them at the moment. On the other hand, he may not have craved the panties, but he still wanted them back just to have the ability to make wishes.
Not only that, he was still female from the waist down. After all, he hadn't ever given the panties to anyone, which would end that. Rather, they were stolen.
Of course, he was only about to learn that they were stolen. Jon finished his shower and headed for his room. He realized that he didn't know if the bodily change caused by the panties changes reality or not--the other day he had changed back as soon as he gave the panties to Karyn, and he only changed to half female again just now. So Jon looked around his room for signs that reality had changed so he was half female all along... and realized the panties weren't there.
He stopped searching and immediately tried to find out who had taken him. The window was still closed and locked. Karyn wouldn't be here to visit at this time. So it was probably a family member. He went down the hall, having had a guess as to who might steal them, and opened one door. To see his kid sister Marie wearing the panties.
Idly, Jon wondered if, assuming the panties do change reality, his kid sister had been a boy once and he just didn't remember it. After all, making the lower half female on someone before puberty would just make them a normal female.